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Questions 2.6f

What is the meaning of a mixture?



Answers to Questions 2.6f

  1. 1. Cut green leaves into small pieces and crush them in a mortar, adding a little acetone (or ethanol) at a time.
    • Filter the mixture and retain the filtrate.
    • Place a drop of the filtrate at the centre of a filter paper held horizontally on a petri dish. Continue to add the filtrate dropwise at the same point (centre), each time allowing it to spread.
    • Allow the paper to dry. The coloured rings obtained are the different substances that were in the leaf juice. How can we obtain them from here?
  2. Filtration. Chromatography begins with setting up the chromatography paper, whether horizontally or vertically, then applying leaf juice on the paper.
  3. Chromatography paper has narrow holes that allow solvent to spread (or rise) by capillarity, carrying the coloured substances with it.